A Phosphite Dehydrogenase Variant with Promiscuous Access to Nicotinamide Cofactor Pools Sustains Fast Phosphite-Dependent Growth of Transplastomic Chlamydomonas reinhardtii
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چکیده
منابع مشابه
Relaxing the nicotinamide cofactor specificity of phosphite dehydrogenase by rational design.
Homology modeling was used to identify two particular residues, Glu175 and Ala176, in Pseudomonas stutzeri phosphite dehydrogenase (PTDH) as the principal determinants of nicotinamide cofactor (NAD(+) and NADP(+)) specificity. Replacement of these two residues by site-directed mutagenesis with Ala175 and Arg176 both separately and in combination resulted in PTDH mutants with relaxed cofactor sp...
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There are a number of turf fungicides the activity of which is based on the phosphite (=phosphonate) ion (P03-). Chipco Signature fungicide (active ingredient fosetyl-Al) is the most common, and has become a "keystone" product for disease control programs on many golf courses across the country. This is in part because of the general enhancement in quality on stressed turfgrass that has been re...
متن کاملEfficient regeneration of NADPH using an engineered phosphite dehydrogenase.
The in situ regeneration of reduced nicotinamide cofactors (NAD(P)H) is necessary for practical synthesis of many important chemicals. Here, we report the engineering of a highly stable and active mutant phosphite dehydrogenase (12x-A176R PTDH) from Pseudomonas stutzeri and evaluation of its potential as an effective NADPH regeneration system in an enzyme membrane reactor. Two practically impor...
متن کاملFurther improvement of phosphite dehydrogenase thermostability by saturation mutagenesis.
Phosphite dehydrogenase represents a new enzymatic system for regenerating reduced nicotinamide cofactors for industrial biocatalysis. We previously engineered a variant of phosphite dehydrogenase with relaxed cofactor specificity and significantly increased activity and stability. Here we performed one round of random mutagenesis followed by comprehensive saturation mutagenesis to further impr...
متن کاملtert-Butylaminium phosphite
In the title compound, C(4)H(12)N(+)·H(2)PO(3) (-), the components are linked by inter-molecular N-H⋯O and O-H⋯O hydrogen bonds, resulting in a two-dimensional framework.
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ژورنال
عنوان ژورنال: Plants
سال: 2020
ISSN: 2223-7747
DOI: 10.3390/plants9040473